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KMID : 0351619700110020303
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Kyungpook Medical Journal
1970 Volume.11 No. 2 p.303 ~ p.313
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³H-thymidine Autoradiographic Cell Cycle Studies of Several Regions in Pre and Postanatal Mouse Kidney
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Abstract
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Recent studies have shown that thymidine, a DNA precursor, is selectively taken up by cells synthesizing new DNA in preparation for cell division, The use of tritiated thymidine for labeling cells in DNA synthesis combined with autoradiography has provided a powerful tool for the study of cell proliferation. Further. since DNA is metabolically stable, cells once labeled can be followed in their migrations and even though changes of cell type.
After injection into animals tritiated thymidine is distributed by the blood stream, and cells which are undergoing DNA synthesis at the time of administration remain identifiable as labeled cells.
In addition, inasmuch as the labeled thymidine of these cells will halve with each subsequent mitotic division, it becomes possible to determine the average duration of the intermitotic interval of these cells within a given period of time.
In a cell population which constantly is being renewed, individual cells divide periodically. This process of mitosis and interphase (periods between cell divisions) is termed the cell cycle.
Toward the end of iuterphase, DNA is synthesized, this being called the DNA duplication or the S stage. After the S stage, the cell enters a relatively quiescent period prior to mitosis called the postduplication or G2 stage and then passes through prophase, metaphase, anaphase and telophase. At the termination of G1 stage of interphase, which lasts until DNA duplication occurs prior to the succeeding mitosis.
In the present study, age changes in these four features of ^(3)H-thymidine labeling were examined in the mouse kidney.
Mice of five ages, 14th day of gestation to 20 day postnatal, were given a single injection of ^(3)H-thymidine and the changes with age in the proportion of cells synthesizing DNA synthesis at the time of injection examined in several histological regions of the kidney such as renal corpuscle, convoluted tubule, and collecting tubule because, embryologlically, the independent origin of the nephrons and the collecting tubules in the embryo has teen mentioned.
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